Adeno-associated virus 3 (ATCC® VR-681)

Classification: Parvoviridae, Dependovirus  /  Product Format: frozen

Permits and Restrictions

View Permits

Classification Parvoviridae, Dependovirus
Agent Adeno-associated virus 3
Strain H
Common Name AAV-3H
Applications
Cross-reacts with AAV-2; no hemagglutinin detected.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions  -70°C or colder
Comments
Requires co-infection with helper adenovirus Adenovirus type 2 (ATCC VR-846) for replication. Helper virus may be inactivated after harvest in water bath at 56°C for 30 minutes. Cross-reacts with AAV-2; no hemagglutinin detected.
Effect on Host
AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
Recommended Host
Production Host: HEK-293 (ATCC CRL-1573).

Production Host:  HEK-293 (ATCC® CRL-1573)

Alternate Hosts: HEK cells, KB cells (ATCC CCL-17) and HeLa cells (ATCC CCL-2)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with helper adenovirus and a small volume (e.g. 1 mL per 25 cm2) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2-3 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
Name of Depositor MD Hoggan
Chain of Custody
Deposited as Adeno-associated virus type 3
Source
Adenovirus 7, SV-40 hybrid E 46 plus strain infected HEK cells
References

Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation