Adeno-associated virus 4 (ATCC® VR-646)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus-4  /  Product Format: frozen

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Classification Parvoviridae, Dependovirus, Adeno-associated virus-4
Deposited As Adeno-associated virus type 4
Agent Adeno-associated virus 4
Strain none
Positive for Mycoplasma Yes
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Monkey cells infected with SV-15 virus
Product Format frozen
Storage Conditions -70°C or colder
Comments
Depends upon adenovirus for replication. *Helper virus may be inactivated at 56C for 30 min.
Effect on Host
Cytopathic effect: AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
AAV produces no CPE.  Helper adenovirus induces refractile rounding and cell sloughing. 
Non-cytopathic without helper virus
Recommended Host
monkey kidney cells; suckling mouse

Recommended Host:  LLC-MK2 (ATCC® CCL-7.1TM)

Growth Conditions
Temperature: 37.0°C
Duration: Evidence of virus growth can be expected within 2-3 days. For best results cells should be 24-48 hours old and 80%-90% confluent (not 100%) at the time of infection.
Effect on Host
Cytopathic effect: AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
AAV produces no CPE.  Helper adenovirus induces refractile rounding and cell sloughing. 
Non-cytopathic without helper virus
Name of Depositor WP Parks
Source
Monkey cells infected with SV-15 virus
References

Chiorini JA, et al. Cloning of adeno-associated virus type 4 (AAV4) and generation of recombinant AAV4 particles. J. Virol. 71: 6823-6833, 1997. PubMed: 9261407

Parks WP, et al. Physical assay and growth cycle studies of a defective adeno-satellite. J. Virol. 1: 171-180, 1967. PubMed: 4990036

Cross References

Nucleotide (GenBank) : U89790 Adeno-associated virus 4, complete genome.

Nucleotide (GenBank) : NC_001829 Adeno-associated virus 4, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation