Adeno-associated virus 1 (ATCC® VR-645)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus-1  /  Product Format: frozen

Classification Parvoviridae, Dependovirus, Adeno-associated virus-1
Deposited As Adeno-associated (satellite) virus type 1
Agent Adeno-associated virus 1
Strain none
Applications
Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Pool of RhMk TC infected with SV-15 virus
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Depends upon adenovirus for replication. Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. *Helper virus may be inactivated by heating to 56C for 30 min.
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  HEK-293 (ATCC® CRL-1573TM)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 2-4 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Name of Depositor WP Parks
Special Collection ATCC
Source
Pool of RhMk TC infected with SV-15 virus
References

Atchison RW, et al. Adenovirus-associated defective virus particles. Science 149: 754-756, 1965. PubMed: 14325163

or colder

or colder