Gallid herpesvirus 2 (ATCC® VR-585)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen 1.0 mL

Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus type 1
Agent Gallid herpesvirus 2
Strain JM
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions Vapor phase of liquid nitrogen
Images VR-585 images
Comments

Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens.

Stable for at least 30 years in viable cells frozen at -196°C.

Antigens detectable by immunodiffusion and immunofluorescence.  Antigenically related to herpesvirus of turkeys (HVT), but distinct from infectious laryngotracheitis and duck enteritis virus.

Proportion of viral DNA composed of guanine and cytosine is 56-57%.
Effect on Host
CPE, cell rounding and enlargement with some syncytia
Recommended Host
Duck embryo cells (ATCC® CCL-141™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed by adding infected cells to freshly dissociated cells and planting simultaneously.
Incubation: 6-14 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and enlargement with some syncytia
Name of Depositor RL Witter
Special Collection ATCC
Source Chicken tumor; blood
References

Sevoian M, Chamberlain DM, and Counter, FT. Avian lymphomatosis. 1. Experimental reproduction of the neural and visceral forms. Vet Med 57: 500-501, 1962.

Solomon JJ, et al. Studies on the etiology of Marek's disease. I. Propagation of the agent in cell culture. Proc. Soc. Exp. Biol. Med. 127: 173-177, 1968. PubMed: 5644639

Stephens EA, et al. Characteristics of JMV Marek's disease tumor: a nonproductively infected transplantable cell lacking in rescuable Virus. J. Natl. Cancer Inst. 57: 865-874, 1976. PubMed: 187779