Japanese encephalitis virus (ATCC® VR-343)

Classification: Flaviviridae, Flavivirus  /  Product Format: freeze-dried

Classification Flaviviridae, Flavivirus
Agent Japanese encephalitis virus
Common Name Japanese encephalitis virus
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Culex tritaeniorhynchus from Japan, 1948
Product Format freeze-dried
Storage Conditions -60°C
Effect on Host
Cytopathic effects in tissue culture
Recommended Host
hamster kidney cells
forms plaques in chicken embryo in tissue culture and double agar overlay in hamster kidney cells; suckling mouse, i.c. inoculation
Growth Conditions
Incubation: 10 days at 24°C; 6 days at 30°C
Effect on Host
Cytopathic effects in tissue culture
Name of Depositor WM Hammon
Source
Culex tritaeniorhynchus from Japan, 1948
Year of Origin 1948
References

Hammon WM. Am. J. Hyg. 50: 51, 1949.

Rohitayodhin S, Hammon WM. Studies on Japanese B encephalitis virus vaccines from tissue culture. II. Development of an attenuated strain of virus. J. Immunol. 89: 589-597, 1962. PubMed: 13982456

Rohitayodhin S, Hammon WM. Studies on Japanese B encephalitis virus vaccines from tissue culture. III. Further selection and testing of attenuated virus lines from OCT-541. J. Immunol. 89: 823-833, 1962. PubMed: 13982454

Hammon WM, et al. Studies on Japanese B encephalitis virus vaccines from tissue culture. IV. Preparation and characterization of pool of attenuated OCT-541 lind for human vaccine trial. J. Immunol. 91: 295-305, 1963. PubMed: 14071019