Hepatitis A virus (ATCC® VR-2266™) Classification: Picornaviridae, Hepatovirus / Product Format: frozen General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Classification Picornaviridae, Hepatovirus Agent Hepatitis A virus Strain LSH/S Applications vaccine productionEnteric Research Biosafety Level 2 Product Format frozen Storage Conditions -70°C or colder Effect on Host No CPE produced. Presence of virus determined by immunofluorescence or by double sandwich ELISA assay. No CPE produced. Presence of virus determined by immunofluorescence or by double sandwich ELISA assay. Recommended Host MRC-5 (ATCC CCL-171)Production Host: MRC-5 (ATCC® CCL-171) Growth Conditions Temperature: 32°CRecommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 90% confluent. Remove CGM and then wash monolayer once with 1X DPBS (ATCC 30-2200). Inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in plain DMEM to provide an optimal MOI (e.g. 0.001). Adsorb 2-2.5 hours at 32°C in a humidified 5% CO2 atmosphere, rotating on a roller apparatus at 0.75 RPM. End adsorption by adding virus growth medium.Incubation: 21 days Effect on Host No CPE produced. Presence of virus determined by immunofluorescence or by double sandwich ELISA assay. No CPE produced. Presence of virus determined by immunofluorescence or by double sandwich ELISA assay. Name of Depositor V Pellegrini, Sclavo S.p.A. Special Collection NCRR Contract Chain of Custody ATCC <<--Sclavo S.p.A.<<--A Zuckermann Source A well documented case of Hepatitis A, London School of Hygiene, 1983 References US Patent 5,719,049 dated Feb 17 1998 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS