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Hepatitis A virus (ATCC® VR-2266™)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen

Classification Picornaviridae, Hepatovirus
Agent Hepatitis A virus
Strain LSH/S
Applications
Vaccine production
Enteric Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Effect on Host
No CPE produced. Presence of virus determined by immunofluorescence or by double sandwich ELISA assay.
Recommended Host
MRC-5 (ATCC® CCL-171™™)
Growth Conditions

Temperature: 32°C

Recommendations for Infection:  Plate cells 24-48 hours prior to infection and infect when cultures are 90% confluent.   Remove CGM and then wash monolayer once with 1X DPBS (ATCC® 30-2200™). Inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in plain DMEM to provide an optimal MOI (e.g. 0.001).  Adsorb 2-2.5 hours at 32°C in a humidified 5% CO2 atmosphere, rotating on a roller apparatus at 0.75 RPM. End adsorption by adding virus growth medium.

Incubation:  Incubate infected culture for 21 days at 32°C in a humidified 5% CO2 atmosphere on a roller apparatus at 0.75 RPM.
Effect on Host
No CPE produced. Presence of virus determined by immunofluorescence or by double sandwich ELISA assay.
Name of Depositor V Pellegrini, Sclavo S.p.A.
U.S. Patent 5,607,851
5,719,049
U.S. Patent Number 5,607,851
5,719,049
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Special Collection NCRR Contract
Chain of Custody
ATCC <-- Sclavo S.p.A. <-- A Zuckermann
Source A well documented case of Hepatitis A, London School of Hygiene, 1983
References

US Patent 5,719,049 dated Feb 17 1998