Kilham rat virus (ATCC® VR-1790)

Classification: Parvoviridae, Parvovirus  /  Product Format: 1 mL per vial

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Classification Parvoviridae, Parvovirus
Agent Kilham rat virus
Strain Kilham (RV-13)
Biosafety Level 2
Product Format 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1790 images
Comments Derived by adaptation of primary cell culture product ATCC® VR-235 to NRK (ATCC® CRL-6509) cells. ATCC® VR-235 and ATCC® VR-1790have not been compared for sequence or infectivity in primary cell culture and tissue culture. The virus produces acute fatal disease, stunted growth, or latent infection in hamsters (i.c.), depending on dose of virus and age of animals. Spontaneous transmission from infected to uninoculated hamsters may occur. Virus from tissues of cell cultures will agglutinate guinea pig red blood cells at room temperature. Morphology resembles that of polyoma virus.
Effect on Host CPE, cell rounding, degeneration, cytoplasmic bridging
Recommended Host NRK (ATCC® CRL-6509™)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 40-60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 2-7 days
Effect on Host CPE, cell rounding, degeneration, cytoplasmic bridging
Name of Depositor L Kilham, Dartmouth Medical School
Source Tissues of tumor-bearing rats
References

Kilham L, Olivier LJ. A latent virus of rats isolated in tissue culture. Virology 7: 428-437, 1959. PubMed: 13669314

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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