Parainfluenza 3 (ATCC® VR-1782)

Classification: Paramyxoviridae, Paramyxovirus  /  Product Format: frozen 1 mL per vial

Classification Paramyxoviridae, Paramyxovirus
Agent Parainfluenza 3
Strain ATCC-2011-5
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions  -70°C or colder
Effect on Host
CPE, refractile cell rounding in culture
CPE, refractile cell rounding
Recommended Host

Production Host:  LLC-MK2 Derivative (ATCC® CCL-7.1)

LLC-MK2 Derivative (ATCC CCL-7.1)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium, rinse with plain EMEM, and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 9-14 days in a humidified 5% CO2 atmosphere
Effect on Host
CPE, refractile cell rounding in culture
CPE, refractile cell rounding
Name of Depositor ATCC
Source
Clinical sample from a patient positive for PIV 3 in Virginia, June 2011