Rickettsia tamurai (ATCC® VR-1594)

Classification: Rickettsiaceae, Rickettsieae  /  Product Format: frozen

Permits and Restrictions

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Classification Rickettsiaceae, Rickettsieae
Agent Rickettsia tamurai
Strain AT-1
Positive for Mycoplasma Yes
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -80°C or colder  
The AT-1 strain is recognized as the type strain for a newly identified rickettsial species (Rickettsia tamurae), a gram -negative, obligately intracellular and non-motile bacterium.
The preparation available through ATCC tested positive for mycoplasma (Mycoplasma orale) contamination by PCR.
Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling.
Effect on Host
Bacteria visible in cells after staining with Gimenez stain.
Recommended Host
Vero cells (ATCC CRL-1587)
Growth Conditions
Recommendations for Infection: 5 days at 32°C, a 5% CO2 in air atmosphere is recommended in EMEM (ATCC 30-2003); with 2mM L-glutamine supplemented with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C
Effect on Host
Bacteria visible in cells after staining with Gimenez stain.
Name of Depositor Dr. P. -E. Fournier
Isolated from Amblyomma testudinarium nymph in Anan, Tokushima prefecture, Japan, in 1993

Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804

Fournier, P. E. et. al. Rickettsia tamurae sp. nov., isolated from Amblyomma testudinarium ticks. Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1673-75. PubMed: 16825648

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation