Canine distemper virus (ATCC® VR-1587)

Classification: Paramyxoviridae, Morbillivirus  /  Product Format: frozen

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Classification Paramyxoviridae, Morbillivirus
Agent Canine distemper virus
Strain Snyder Hill (cell culture adapted)
Applications
It is used primarily as a challenge strain to test dogs for immunity to the disease.
ATCC VR-526, deposited by L. E. Carmichael, was originally isolated from the brain of a dog with clinical distemper in September, 1950. This virus produces neurological signs with regularity only when inoculated intracerebrally.
It does not produce hemagglutinin or hemolysin.
Respiratory research
Positive for Mycoplasma No
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments Neurological signs are produced with regularity only when inoculated intracerebrally. This strain does not produce hemagglutinin or hemolysin. It is used primarily as a challenge strain to test dogs for immunity to this disease. Cross-immunity tests in dogs have shown it to be similar to other classical strains of CDV.
Effect on Host
CPE, vacuolization and rounding of cells, sloughing
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 90% confluent (not 100% confluent).

Incubation: 5 to 7 days at 37°C, a 5% CO2 in air atmosphere is recommended

Effect on Host
CPE, vacuolization and rounding of cells, sloughing
Name of Depositor LE Carmichael, ATCC
Special Collection ATCC
Source ATCC® VR-1587™ was derived at ATCC from ATCC® VR-526™ by adaptation to growth in Vero cells (ATCC® CCL-81™). ATCC® VR-526™, deposited by L.E. Carmichael, was isolated from brain of dog with clinical distemper, September, 1950. Because it was propagated in animal tissue ATCC will no longer offer that product.
References

Gillespie JH. . Am. J. Vet. Res. 17: 103, 1956. PubMed: 13259024

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation