Gallid herpesvirus 2 (ATCC® VR-1576)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Permits and Restrictions

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Classification Herpesviridae, Mardivirus
Agent Gallid herpesvirus 2
Strain 648A
Applications
Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO. Controlled rate freezing is recommended.
Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium.
With strains JM (v pathotype; ATCC VR-585) and Md-5 (vv pathotype; ATCC VR-987) can be used to classify new viral strains for pathotype.
Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines.
Positive for Mycoplasma No
Biosafety Level 2
Isolation
Isolated from chicken blood lymphocytes
Product Format frozen
Storage Conditions  Vapor phase of liquid Nitrogen
Comments
Set up cells 24 to 48 hours in advance. Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium. Adsorption is not required. Incubate 4-7 days at 38° C in a humidified 5% CO2 atmosphere. Harvest by trypsinizaion of infected cell culture. Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO. Controlled rate freezing is recommended.
Widely recognized representative of the MDV vv+ pathotype. With strains JM (v pathotype; ATCC VR-585) and Md-5 (vv pathotype; ATCC VR-987) can be used to classify new viral strains for pathotype. Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines. Also infects turkeys and quail. The virus is highly cell associated and must be propagated and stored as viable infected cells. A stock of infected cells at high density (107/ml) typically has an infectivity titer of about 106 PFU/ml.
Effect on Host
Infection produces focal CPE in cell culture (plaques) consisting of rounded cells and polykaryocytes. In chickens, induces lymphoproliferative lesions (tumors) in peripheral nerves and viscera which cause paralysis and death.
Effects:  Infection produces focal CPE in cell culture (plaques) consisting of rounded cells and polykaryocytes.  In chickens, induces lymphoproliferative lesions (tumors) in peripheral nerves and viscera which cause paralysis and death.
Recommended Host

Recommended Host:  Duck or chick embryo fibroblast cultures (e. g. ATCC® CRL-1590™)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 38.0°C
Duration: 4-7 days required for infection. Virus is highly cell associated and must be preserved in the form of viable cryopreserved cells.
Cryopreservation
Freeze medium: Culture medium containing 10% serum and 10% DMSO. Controlled rate freezing is recommended.
Storage temperature: Liquid Nitrogen
Effect on Host
Infection produces focal CPE in cell culture (plaques) consisting of rounded cells and polykaryocytes. In chickens, induces lymphoproliferative lesions (tumors) in peripheral nerves and viscera which cause paralysis and death.
Effects:  Infection produces focal CPE in cell culture (plaques) consisting of rounded cells and polykaryocytes.  In chickens, induces lymphoproliferative lesions (tumors) in peripheral nerves and viscera which cause paralysis and death.
Name of Depositor RL Witter
Special Collection ATCC
Source
Isolated from chicken blood lymphocytes
References

Witter RL. Increased virulence of Marek's disease field isolates. Avian Dis. 41: 149-163, 1997. PubMed: 9087332

Dudnikova E, et al. Evaluation of Marek's disease field isolates by the "best fit" pathotyping assay. Avian Pathology 36(2):135-143, 2007.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation