Hepatitis A virus (ATCC® VR-1541)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Picornaviridae, Hepatovirus
Agent Hepatitis A virus
Strain HM 175 (clone 1)
Applications
Enteric Research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration
Recommended Host FRhK4 (ATCC® CRL-1688™)
Growth Conditions Temperature: 34.5°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34.5°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 14-28 days
Effect on Host No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration
Name of Depositor RH Purcell, National Institute of Allergy and Infectious Diseases; ATCC
Special Collection NCRR Contract
Source Human stool specimens from an outbreak of the virus, Australia.
References

Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685

Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation