Hepatitis A virus (ATCC® VR-1541)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen 1 mL per vial

Classification Picornaviridae, Hepatovirus
Agent Hepatitis A virus
Strain HM 175 (clone 1)
Applications
Enteric Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration
Recommended Host FRhK4 (ATCC® CRL-1688™)
Growth Conditions Temperature: 34.5°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34.5°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 14-28 days
Effect on Host No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration
Name of Depositor RH Purcell, National Institute of Allergy and Infectious Diseases; ATCC
Special Collection NCRR Contract
Source Human stool specimens from an outbreak of the virus, Australia.
References

Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685

Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985