Adeno-associated virus 5 (ATCC® VR-1523)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus 5  /  Product Format: frozen

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Classification Parvoviridae, Dependovirus, Adeno-associated virus 5
Agent Adeno-associated virus 5
Strain Original
Applications
Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells.
Biosafety Level 2
Isolation
clinical specimen - human
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Depends upon adenovirus for replication. Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells. *Helper virus may be inactivated by heating to 56C for 30 min.
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  LLC-MK2 (ATCC® CCL-7.1TM)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 4 - 5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). [Note: requires viral helper for productive infections, e.g., VR-846]
Cryopreservation
Storage temperature: -70°C
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Name of Depositor U Bantel-Schaal, H zur Hausen, U Bantel-Schaal
Special Collection NCRR Contract
Source
clinical specimen - human
References

or colder

Georg-Fries B, et al. Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. Virology 134: 64-71, 1984. PubMed: 6200995

Bantel-Schaal U, zur Hausen H. Characterization of the DNA of a defective human parvovirus isolated from a genital site. Virology 134: 52-63, 1984. PubMed: 6324476

can also be grown on other human and rodent cell lines

penile flat condylomatous lesion

requires viral helper for productive infections

confirm growth with PCR

Bantel-Schaal U, et al. Human adeno-associated virus type 5 is only distantly related to other known primate helper-dependent parvoviruses. J. Virol. 73: 939-947, 1999. PubMed: 9882294

Bantel-Schaal U, et al. Endocytosis of adeno-associated virus type 5 leads to accumulation of virus particles in the Golgi compartment. J. Virol. 76: 2340-2349, 2002. PubMed: 11836412

or colder

Cross References

Nucleotide (GenBank) : AF085716 Adeno-associated virus 5 DNA binding trs helicase (Rep22) and capsid protein (VP1) genes, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation