Mus musculus (ATCC® VR-1518AF-MAB)

Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod  /  Strain: Monoclonal Antibody against FOS peptide(359-378)  /  Product Format: freeze-dried Titer: Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay.

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Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Agent Mus musculus
Strain monoclonal antibody against FOS peptide (359-378) [LA 054]
Common Name mouse
Applications
Source and Sex of Animals: Male 129GIX+ mice were used for immunization.
Cross-reactivity: In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr.
Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay
Biosafety Level 1
Isolation
Source and Sex of Animals: Male 129GIX+ mice were used for immunization.
Product Format freeze-dried Titer: Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay.
Storage Conditions Vials may be stored at 2-8°C.
Comments
Immunogen: Synthetic peptide RKGSSSNEPSSDSLSSPTLL(C) (residues 359-378 of the FOS sequence) coupled to keyhole limpet hemocyanin
Source and Sex of Animals: Male 129GIX+ mice were used for immunization.
Fusion Partners: 1.3 x 10(8) nucleated spleen cells with 2 x 10(7) SP2/0 non-secreting myeloma cells
Cross-reactivity: In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr.
Immunization Schedule: Approximately 70 mg of coupled peptide in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and complete Freund's adjuvant administered intraperitoneally on day 0;
followed by an equal amount of conjugate in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and 10 mg/ml alum administered intraperitoneally on day 14;
followed by 70 mg of coupled peptide in 200 ml of phosphate buffered saline pH 7.4 administered intravenously on day 51. The spleen was removed on day 56.
Preparation: Monoclonal antibody is in ascites fluid collected from 2-06-87 to 7-06-87.
The ascites fluid was ammonium sulfate precipitated, reconstituted, filter sterilized, aliquoted 1 ml per vial, and lyophilized. The sterility is not guaranteed. Resuspend in 1 ml distilled water.
Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay
Vials may be stored at 2-8°C.
IgG1
Effect on Host
Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay
Recommended Host
Source and Sex of Animals: Male 129GIX+ mice were used for immunization.
Cryopreservation
Storage temperature: Vials may be stored at 2-8°C.
Effect on Host
Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation