Ophidian reovirus (ATCC® VR-1496)

Product Format: frozen

Agent Ophidian reovirus
Strain Elaphe 1
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Elaphe species (beauty snakes, and black ratsnakes) necropsied after unexpected death
Product Format frozen
Storage Conditions   -70°C or colder
Comments
does NOT hemagglutinate chicken or guinea pig erythrocytes
-70C or lower
Effect on Host
Yes, in vitro effects: Cytopathic effects (large syncytia formation followed by cell detachement)
CPE (large syncytia formation followed by cell detachment)
Recommended Host
VH2 (ATCC CCL-140)

Host of Choice:  VH2 (ATCC CCL-140)

Host Range:  Moellendorff's ratsnakes, beauty snakes, and black ratsnakes;  VH2 TC

Moellendorff's ratsnakes; beauty snakes; black ratsnakes; VH2 cells (ATCC CCL-140)
Growth Conditions
Duration: 2 to 5 days at 30C in VH2 (ATCC CCL-140)
Cryopreservation
Storage temperature: -70C or lower
Effect on Host
Yes, in vitro effects: Cytopathic effects (large syncytia formation followed by cell detachement)
CPE (large syncytia formation followed by cell detachment)
Name of Depositor EW Lamirande
Chain of Custody
ATCC <<--EW Lamirande<<--J. Gaskin
Source
Elaphe species (beauty snakes, and black ratsnakes) necropsied after unexpected death
References

Lamirande EW, et al. Isolation and experimental transmission of a reovirus pathogenic in ratsnakes (Elaphe species). Virus Res. 63: 135-141, 1999. PubMed: 10509725