Herpesvirus saimiri (ATCC® VR-1396)

Classification: Herpesviridae, Rhadinovirus  /  Product Format: frozen

Classification Herpesviridae, Rhadinovirus
Agent Herpesvirus saimiri
Strain C-488
Common Name Herpesvirus saimiri
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments This item contains FBS of Guatemalan origin. Strain is also available as ATCC® VR-1414™, which does not require a USDA permit due to multiple passages of ATCC® VR-1396™ in US origin FBS.

Herpesvirus saimiri strain C488 transforms human T lymphocytes to antigen- and mitogen-independent, but IL-2 dependent stable growth. Virus stocks are stable for months at 4C, but titer greatly reduced by freeze-thaw cycles.

For methods of propagation see: Fickenscher and Fleckenstein, 1994. in: Methods in Molecular Genetics. (ed) K. W. Adolph, 4: 345-362, 1994.
Effect on Host
CPE, foci of detached cells bordered by rounded enlarged cells
Recommended Host
OMK (ATCC® CRL-1556™)
Growth Conditions
Temperature: 37°C

Incubation:  Incubate infected culture for 10-20 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance

Effect on Host
CPE, foci of detached cells bordered by rounded enlarged cells
Name of Depositor M Daniel, R Desrosiers, B Biesinger, B Fleckenstein, H Fickenscher
Special Collection NCRR Contract
Source Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus)
References

Biesinger B, et al. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology 176: 505-514, 1990. PubMed: 2161148

Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J. Virol. 43: 352-356, 1982. PubMed: 6287011

Biesinger B, et al. Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proc. Natl. Acad. Sci. USA 89: 3116-3119, 1992. PubMed: 1313581

. Methods in Molecular Genetics, Part A. : K.W. Adolph; 1993.