Chlamydophila pneumoniae (ATCC® VR-1355)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen

Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae Grayston et al.
Agent Chlamydophila pneumoniae
Strain TWAR strain 2043
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
Forms intracellular inclusions which react with specific anti-C. pneumoniae monoclonal antibodies and monoclonal antibodies directed against the chlamydia LPS (genus antigen: Kallestad C. trachomatis Culture Confirmation System).
Will not stain with iodine or monoclonal antibodies against the MOMP of C. trachomatis. Infection is enhanced by centrifugation and use of cycloheximide (1-2 µg/mL) in medium.
This preparation has tested positive for Mycoplasma contamination.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Recommended Host
HEp-2 cells (ATCC® CCL-23™)
Growth Conditions

Temperature: 35°C

Incubation: 3 days at 35°C, a 5% CO2 in air atmosphere is recommended

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90% - 100% confluent.

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Name of Depositor MR Hammerschlag
Source Nasopharynx of human patient with pneumonia
References

Chirgwin K, et al. In vitro susceptibilities of Chlamydia pneumoniae (Chlamydia sp. strain TWAR). Antimicrob. Agents Chemother. 33: 1634-1635, 1989. PubMed: 2817862