MEF (CF-1) IRR (ATCC® SCRC-1040.1™) Organism: Mus musculus, mouse / Cell Type: Fibroblast / Tissue: Embryo / General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Mus musculus, mouse Tissue Embryo Cell Type Fibroblast Product Format frozen Morphology Fibroblast Culture Properties Adherent Biosafety Level 1 Age 14 days gestation embryo Gender male and female mixed Strain CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms Applications Irradiated cells for use as feeder layer Storage Conditions liquid nitrogen vapor phase Clinical Data male and female mixed Comments These cells have been growth-arrested by irradiation with 10,00 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days. Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended). Subculturing Medium Renewal: Twice a week or when pH decreases Culture Conditions Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C Name of Depositor ATCC Year of Origin 2004 References Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.