Acanthamoeba hatchetti Sawyer et al. (ATCC® PRA-115)

Strain Designations: 4RE  /  Depositor: J Walochnik  /  Biosafety Level: 2

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Strain Designations 4RE
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
clinical specimen - human
Graz Austria
Isolation date: 1997
Product Format frozen
Storage Conditions liquid nitrogen vapor phase
Type Strain no
Medium ATCC® Medium 712: PYG w/ Additives
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
Interval: every month
Subcultivation
Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
Interval: every month
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Name of Depositor J Walochnik
Chain of Custody
J Walochnik
Year of Origin 1997
References

Walochnik J, et al. Discrimination between clinically relevant and nonrelevant Acanthamoeba strains isolated from contact lens- wearing keratitis patients in Austria. J. Clin. Microbiol. 38: 3932-3936, 2000. PubMed: 11060047

Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891

isolated from the contact lens case of a patient with non-acanthamoeba keratoconjunctivitis

Cross References

Nucleotide (GenBank) : AF019068 Acanthamoeba hatchetti BH-2 18S ribosomal RNA gene, partial sequence

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation