Herpetomonas muscarum muscarum Rogers and Wallace (ATCC® 30260)

Depositor: FG Wallace, WE Rogers  /  Biosafety Level: 1

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Phylogenetic analysis of RNA editing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Musca domestica, Minneapolis, MN, 1968
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain yes
species description
Glucose catabolism
Effect of platelet-activationg factor on development
species description
Phylogenetic analysis of RNA editing
Proteolytic activities
Acetylornithinase and ornithine acetyltransferase
Ultrastructural differences between species with and without endosymbionts
Unique no-branched polyunsaturated fatty acids
isoleucine requirement and threonine deaminase
Monoclonal antibodies for identification
Cyclopropane fatty acid
endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification
Medium ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 1012: Diphasic blood agar medium
ATCC® Medium 947: L diphasic blood agar (ATCC Medium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 ml
Growth Conditions
Temperature: 25°C
Culture System: Axenic
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).  The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh tube of ATCC medium 1011. 
  10. Incubate vertically at 25°C with the cap screwed on tightly.
  11. Maintain as described above. 
Name of Depositor FG Wallace, WE Rogers
Year of Origin 1968

Rogers WE, Wallace FG. Two new subspecies of Herpetomonas muscarum (Leidy, 1856) Kent, 1880. J. Protozool. 18: 645-654, 1971. PubMed: 4109037

Yoshida N. Herpetomonas mariadeanei sp. n. (Protozoa, Trypanosomatidae) from Muscina stabulans (Fallen, 1816) (Diptera, Muscidae). J. Protozool. 25: 421-425, 1978.

Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148

Van der Ploeg LH, et al. Chromosomes of kinetoplastida. EMBO J. 3: 3109-3115, 1984. PubMed: 6526012

Redman CA, Coombs GH. The products and pathways of glucose catabolism in Herpetomonas muscarum ingenoplastis and Herpetomonas muscarum muscarum. J. Eukaryot. Microbiol. 44: 46-51, 1997.

Lopes AH, et al. Effect of platelet-activating factor on the process of cellular differentiation of Herpetomonas muscarum muscarum. J. Eukaryot. Microbiol. 44: 321-325, 1997. PubMed: 9225446

Coombs GH. Proteinases of Leishmania mexicana and other flagellate protozoa. Parasitology 84: 149-155, 1982. PubMed: 6460959

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989.

Landweber LF, Gilbert W. Phylogenetic analysis of RNA editing: a primitive genetic phenomenon. Proc. Natl. Acad. Sci. USA 91: 918-921, 1994. PubMed: 8302867

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533

Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925

type strain

Cross References

Nucleotide (GenBank) : U01007 Herpetomonas muscarum muscarum kinetoplast 9S rRNA gene.

Nucleotide (GenBank) : U01011 Herpetomonas muscarum muscarum kinetoplast 12S rRNA gene.

Nucleotide (GenBank) : U01015 Herpetomonas muscarum muscarum 16S-like small subunit rRNA.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation