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PRA-177 ^™

Perkinsus honshuensis (ATCC^® PRA-177^™)

Strain Designations: Mie-3G/H8 / Depositor: C Dungan / Biosafety Level: 2

Perkinsus honshuensis ATCC^® PRA-177^™

frozen

For-Profit: $354.00 Non-Profit: $300.90

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Permits and Restrictions	View Permits
Strain Designations	Mie-3G/H8
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	marine invertebrate Mie, Japan Isolation date: May 29, 2002
Product Format	frozen
Type Strain	yes

Comments	This strain is the holotype representative of Perkinsus honshuensis. This species was isolated from gill tissue from the Manila Clam, Venerupis ( = Tapes, Ruditapes) philippinarum.

Medium	ATCC^® Medium 1886: Perkinsus broth medium
Growth Conditions	Temperature: 25.0°C Max Temperature: 28.0°C Min Temperature: 25.0°C Duration: axenic Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Subcultivation	Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.

Name of Depositor	C Dungan
Special Collection	NSF - Protistology
Geographical Isolation	Gokasho Bay
Year of Origin	May 29, 2002
References	Synonym of Perkinsus olseni Lester & Davis, 1981 and P. atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus.. Bull. Eur. Ass. Fish Pathol. 22: 258-265, 2002. Dungan C F, Reece K S. In vitro propagation of two Perkinsus spp. parasites from japanese manila clams Venerupis philippinarum, and description of Perkinsus honshuensis n. sp.. J. Eukaryot. Microbiol. in press: 2006..
Cross References	Nucleotide (GenBank) : DQ516680 LSU rRNA Nucleotide (GenBank) : DQ516681 LSU rDNA Nucleotide (GenBank) : DQ516682 LSU rDNA Nucleotide (GenBank) : DQ516684 LSU rDNA Nucleotide (GenBank) : DQ516699 ITS rDNA Nucleotide (GenBank) : DQ516700 ITS rDNA Nucleotide (GenBank) : DQ516701 ITS rDNA Nucleotide (GenBank) : DQ516702 ITS rDNA Nucleotide (GenBank) : DQ516686 Actin gene Nucleotide (GenBank) : DQ516687 Actin gene Nucleotide (GenBank) : DQ516688 Actin gene Nucleotide (GenBank) : DQ516689 Actin gene Nucleotide (GenBank) : DQ516690 Actin gene Nucleotide (GenBank) : DQ516691 Actin gene Nucleotide (GenBank) : DQ516692 Actin gene

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS