Trypanosoma cruzi Chagas (ATCC® 50794)

Strain Designations: M/HOM/BR/77/ESMERALDO CL3  /  Depositor: JA Dvorak  /  Biosafety Level: 2

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Strain Designations M/HOM/BR/77/ESMERALDO CL3
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

derived from strain Esmeraldo, originally isolated by xenodiagnosis from an acute case of Chagas' disease in a human male from northeastern Brazil, 1977, cloned by M. Miles
Product Format frozen
Type Strain no
identification of isoenzyme patterns
correlation of growth kinetics to zymodeme type
flow cytometric analysis
Brazilian and Bolivian zymodemes
isoenzyme profile
Medium ATCC® Medium 1029: LIT medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
1.   Harvest cells from several cultures in very late logarithmic to early stationary phase of growth.  Vigorously agitate to suspend the cells.

2.   Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.

3.   Centrifuge at ~800 x g for 5 min.

4.   While cells are centrifuging, prepare a 10% solution of DMSO in complete ATCC Medium 1029.  Cool on ice.

5.   Remove the supernatant and pool the cell pellets to the final volume desired with fresh growth medium.

6.   Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.

7.   Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).

8.   Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  At -40°C, plunge ampules into liquid nitrogen.  Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.).  

9.   Store ampules in a liquid nitrogen refrigerator until needed.

10.          To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.  Allow the ampule to thaw completely (2-3 min).

11.          Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh complete ATCC medium 1029.

12.Screw the cap on tightly and incubate at 20-25°C.    Observe the culture daily and transfer when numerous trophozoites are observed.         

Name of Depositor JA Dvorak
Special Collection NCRR Contract
Chain of Custody
ATCC <<--JA Dvorak<<--M.A. Miles
Year of Origin 1977

Miles MA, et al. The identification by isoenzyme patterns of two distinct strain-groups of Trypanosoma cruzi, circulating independently in a rural area of Brazil. Trans. R. Soc. Trop. Med. Hyg. 71: 217-225, 1977. PubMed: 407674

Dvorak JA, et al. Trypanosoma cruzi: correlation of growth kinetics to zymodeme type in clones derived from various sources. J. Protozool. 27: 472-474, 1980.

Dvorak JA, et al. Trypanosoma cruzi: flow cytometric analysis. I. Analysis of total DNA/organism by means of mithramycin-induced fluorescence. J. Protozool. 29: 430-437, 1982. PubMed: 6182288

Tibayrenc M, Miles MA. A genetic comparison between Brazilian and Bolivian zymodemes of Trypanosoma cruzi. Trans. R. Soc. Trop. Med. Hyg. 77: 76-83, 1983. PubMed: 6344363

Chapman MD, et al. Trypanosoma cruzi from the Paraguayan Chaco: isoenzyme profiles of strains isolated at Makthlawaiya. J. Protozool. 31: 482-486, 1984. PubMed: 6239030

Miyahira Y, Dvorak JA. Kinetoplastidae display naturally occurring ancillary DNA-containing structures. Mol. Biochem. Parasitol. 65: 339-349, 1994. PubMed: 7969274

Nozaki T, et al. Cellular and molecular biological analyses of nifurtimox resistance in Trypanosoma cruzi. Am. J. Trop. Med. Hyg. 55: 111-117, 1996. PubMed: 8702014

McDaniel JP, Dvorak JA. Identification, isolation, and characterization of naturally-occurring Trypanosoma cruzi variants. Mol. Biochem. Parasitol. 57: 213-222, 1993. PubMed: 8433713

Finley RW, Dvorak JA. Trypanosoma cruzi: analysis of the population dynamics of heterogeneous mixtures. J. Protozool. 34: 409-415, 1987. PubMed: 3323478

Bongertz V, Dvorak JA. Trypanosoma cruzi: antigenic analysis of cloned stocks. Am. J. Trop. Med. Hyg. 32: 716-722, 1983. PubMed: 6349399

Nozaki T, Dvorak JA. Intraspecific diversity in the response of Trypanosoma cruzi to environmental stress. J. Parasitol. 79: 451-454, 1993. PubMed: 8501607

Nozaki T, Dvorak JA. Trypanosoma cruzi: flow cytometric analysis of developmental stage differences in DNA. J. Protozool. 38: 234-243, 1991. PubMed: 1880761

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation