Leptomonas costoris Wallace et al. (ATCC® 30262)

Strain Designations: [ATCC 30210]  /  Depositor: FG Wallace  /  Biosafety Level: 1

Strain Designations [ATCC 30210]
Biosafety Level 1
Isolation
Gerris comatus, Lake Minnetonka, MN, 1965
Product Format frozen
Type Strain yes
Comments
species description
Cyclopropane fatty acid
Medium ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace
Year of Origin 1965
References

Wallace. Flagellate parasites of water striders with a description of Leptomonas costoris, s. sp.. J. Protozool. 12: 390-393, 1965.

Daggett PM, Decker JE. Some physiological alterations accompanying infectivity to mammals by four genera of Trypanosomatidae. Comp. Biochem. Physiol. 59: 363-366, 1978.

Dollahon NR, et al. Herpetomonas megaseliae, Crithidia fasciculata, and Leptomonas collosoma (Kinetoplastida, Trypanosomatidae) in feces of lizards fed culture forms of the flagellates. J. Protozool. 30: 58-62, 1983.

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Kim R, Ray DS. Conservation of a 29-base-pair sequence within maxicircle ARS fragments from six species of trypanosomes. Gene 40: 291-299, 1985. PubMed: 3938423

type strain