Dictyostelium discoideum Raper (ATCC® 28368)

Strain Designations: Ax-3  /  Product Format: frozen

Permits and Restrictions

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Strain Designations Ax-3
produces calmodulin
transformation host
hydrolysis of a phosphonate ester
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Type Strain no
Preceptrol® no
Actin binding to plasma membrane
Thymidine kinase activity
part of Eumycetozoan Project
Medium ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
ATCC® Medium 671: Dictyostelium medium
Growth Conditions
Temperature: 20.0-25.0°C
This culture contains food source bacteria and thus it is not axenic.
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor WF Loomis
Special Collection Part of Eumycetozoan Project
Chain of Custody
ATCC <-- WF Loomis
Cross References

Nucleotide (GenBank) : D12883 gene coding for gp138

Nucleotide (GenBank) : D12884 gene coding for gp138


Loomis WF Jr.. Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Exp. Cell Res. 64: 484-486, 1971. PubMed: 5542656

Yarger J, et al. Observations on the growth of Dictyostelium discoideum in axenic medium: evidence for an extracellular growth inhibitor synthesized by stationary phase cells. J. Cell Sci. 14: 681-690, 1974. PubMed: 4857469

Fey P, et al. Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum. Gene 165: 127-130, 1995. PubMed: 7489901

Kessin RH, et al. Linkage analysis in Dictyostelium discoideum using temperature- sensitive growth mutants selected with bromodeoxyuridine. J. Bacteriol. 119: 776-783, 1974. PubMed: 4859399

Michrina CA, Deering RA. Thymidine kinase activity in Dictyostelium discoideum. J. Gen. Microbiol. 119: 263-266, 1980. PubMed: 7411120

Jacobson BS. Actin binding to the cytoplasmic surface of the plasma membrane isolated from Dictyostelium discoideum. Biochem. Biophys. Res. Commun. 97: 1493-1498, 1980. PubMed: 6894237

Fang H, et al. Molecular cloning and characterization of two genes encoding gp138, a cell surface glycoprotein involved in the sexual cell fusion of Dictyostelium discoideum. Dev. Biol. 156: 201-208, 1993. PubMed: 7680629

Rossomando EF, Jahngen JH. Hydrolysis of a phosphonate ester catalyzed by an enzyme from Dictyostelium discoideum. Arch. Biochem. Biophys. 197: 364-366, 1979. PubMed: 232405

Clarke M, et al. Isolation and properties of calmodulin from Dictyostelium discoideum. J. Bacteriol. 141: 397-400, 1980. PubMed: 6243626

Bazari WL, Clarke M. Characterization of a novel calmodulin from Dictyostelium discoideum. J. Biol. Chem. 256: 3598-3603, 1981. PubMed: 6259174

Loomis WF. Dictyostelium discoideum. A developmental system. Academic Press, New York, 1975.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation