Amphora roettgeri Lee and Reimer (ATCC® 50213)

Depositor: JJ Lee  /  Biosafety Level: 1

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
endosymbiont from foraminiferan, Heterostegina depressa, coral gardens in the northeastern part of Heron-Wistori Channel, southern extremity of the Great Barrier Reef, 1981
Product Format frozen
Type Strain yes
Medium ATCC® Medium 1966: Soil seawater medium for algae
Growth Conditions
Temperature: 25.0°C
Protocol: ATCCNO: 50213 SPEC: Transfer thawed contents of ampule to a centrifuge tube, centrifuge at 300-400 x g for 5 minutes and remove 0.75 ml. Resuspend the cells in approximately 1 ml of fresh medium and transfer to a T-25 tissue culture flask containing 9 ml of fresh medium. Incubate under light intensity of approximately 50-75 uEinsteins/m2/sec. Subculture every month as follows: Rub the surface of the agar with a sterile cotton swab and aseptically transfer a 0.1 ml aliquot to a fresh flask of medium.
Subcultivation
Protocol: ATCCNO: 50213 SPEC: Transfer thawed contents of ampule to a centrifuge tube, centrifuge at 300-400 x g for 5 minutes and remove 0.75 ml. Resuspend the cells in approximately 1 ml of fresh medium and transfer to a T-25 tissue culture flask containing 9 ml of fresh medium. Incubate under light intensity of approximately 50-75 uEinsteins/m2/sec. Subculture every month as follows: Rub the surface of the agar with a sterile cotton swab and aseptically transfer a 0.1 ml aliquot to a fresh flask of medium.
Name of Depositor JJ Lee
Year of Origin 1981
References

type strain