pSR660 (ATCC® MBA-25)

Applications: expression vectorvector component of two-hybrid screening system  /  Depositors: DA Daines

Permits and Restrictions

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Designations pSR660
Depositors DA Daines
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Vector: pSR660 (plasmid)
Type: expression
Construction: pMS604, pTrcHisB
Features: marker(s): tetR
operator: WT LexA
other: reading frame B
promoter for expression: sulA
replicon: ColE1
Applications
expression vector
vector component of two-hybrid screening system
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Daines DA, et al. Use of a LexA-based system to identify protein:protein interactions in vivo. Methods Enzymol. 358: 153-161, 2002. PubMed: 12474385

Daines DA, Silver RP. Evidence for multimerization of neu proteins involved in polysialic acid synthesis in Escherichia coli K1 using improved LexA-based vectors. J. Bacteriol. 182: 5267-5270, 2000. PubMed: 10960115

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