pSR660 (ATCC® MBA-25)

Applications: expression vectorvector component of two-hybrid screening system  /  Depositors: DA Daines

Permits and Restrictions

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Designations pSR660
Depositors DA Daines
Biosafety Level 1
Vector Information
Vector: pSR660 (plasmid)
Type: expression
Construction: pMS604, pTrcHisB
Features: marker(s): tetR
operator: WT LexA
other: reading frame B
promoter for expression: sulA
replicon: ColE1
Applications
expression vector
vector component of two-hybrid screening system
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Daines DA, et al. Use of a LexA-based system to identify protein:protein interactions in vivo. Methods Enzymol. 358: 153-161, 2002. PubMed: 12474385

Daines DA, Silver RP. Evidence for multimerization of neu proteins involved in polysialic acid synthesis in Escherichia coli K1 using improved LexA-based vectors. J. Bacteriol. 182: 5267-5270, 2000. PubMed: 10960115

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