pGEV-TRP1 plasmid in E. coli (ATCC® MBA-173)

Designations pGEV-TRP1 plasmid in E. coli
Permits and Restrictions

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Biosafety Level 1
Vector Information
Size (kb): 7.4450000000000000
Vector: pGEV-TRP1 plasmid in E. coli (plasmid)
Type: expression
Promoters: Eukaryotic Promoter for expression MRP7
Construction: pRS314, MRP7 promoter, GEV (Gal4, B-estradiol activator, VP16)
A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS314. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.