pFa6a-HISMX6-pADH1-3HA (ATCC® 87815)

Applications: cloning vector  /  Depositors: DJ DeMarini

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Designations pFa6a-HISMX6-pADH1-3HA
Depositors DJ DeMarini
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.8340001106262210
Vector: pFa6a-HISMX6-pADH1-3HA (plasmid)
Construction: pCRTOPO2.1
Construct size (kb): 4.834000110626221
Features: marker(s): ampR, HIS
tag (N-terminal): HA tag
cloning vector
Restriction digests of the clone give the following sizes (kb): BglII--4.8; EcoRI--4.8; BglII/BamHI--4.0, 0.8.
The marker, promoter and 3HA tag are flanked by the F4 primer site (5') and the R3 primer site (3'). The size of F4/R3 PCR product is 1969 nt.
R3 primer sequence: 5'(gene-specific sequence)GCACTGAGCAGCGTAATCTG-3'. The gene-specific sequence of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene. The sequences shown here are the reverse 3'.
Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899

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