pFa6a-TRP1-pADH1-3HA (ATCC® 87813)

Applications: cloning vector  /  Depositors: DJ DeMarini

Designations pFa6a-TRP1-pADH1-3HA
Permits and Restrictions

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Depositors DJ DeMarini
Biosafety Level 1
Vector Information
Size (kb): 4.4670000076293950
Vector: pFa6a-TRP1-pADH1-3HA (plasmid)
Construction: pCRTOPO2.1
Marker(s):ampR
Construct size (kb): 4.467000007629395
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--4.5; EcoRI--4.5; SalI/BglII--3.5, 1.0.
The marker, promoter and 3HA tag are flanked by the F4 primer site (5') and the R3 primer site (3'). The size of the F4/R3 PCR product is 1602 nt. F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'.
R3 primer sequence: 5'(gene-specific sequence)GCACTGAGCAGCGTAATCTG-3'. The gene-specific sequence of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene. The sequences shown here are the reverse 3'.
Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899

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