pFa6a-TRP-pADH1 (ATCC® 87810)

Applications: cloning vector  /  Depositors: DJ DeMarini

Designations pFa6a-TRP-pADH1
Permits and Restrictions

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Depositors DJ DeMarini
Biosafety Level 1
Vector Information
Size (kb): 4.1250000000000000
Vector: pFa6a-TRP-pADH1 (plasmid)
Promoters: Promoter ADH1
Construction: pCRTOPO2.1
Construct size (kb): 4.125
Features: marker(s): ampR, TRP1
promoter: ADH1
cloning vector
Restriction digests of the clone give the following sizes (kb): BglII--4.1; EcoRI--4.1; BglII/EcoRI--3.2, 0.9.
The marker and promoter are flanked by the F4 primer site(5') and the R6 primer site (3'). The size of the the F4/R6 PCR product should be 1482nt.
F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.
Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.
Growth Conditions
Temperature: 37.0°C

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899

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