HO-poly-KanMX4-HO (ATCC® 87804)

Applications: Cloning vector  /  Depositors: DJ Stillman

Designations HO-poly-KanMX4-HO
Permits and Restrictions

View Permits

Depositors DJ Stillman
Biosafety Level 1
Vector Information
Construct size (kb): 6.06
Vector: HO-poly-KanMX4-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s): ampR
Features: promoter: HO
Cloning vector
Restriction digests of the clone give the following sizes (kb): NotI--3.0 (doublet); XbaI--6.0; PstI--5.8, 0.2.
The plasmid contains two fragments from the HO promoter inserted in pUC21-NotI polylinker along with the kanMX selectable marker. It allows for targeted integration at HO locus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

David J Stillman, personal communication

Shipped frozen
Shipping Information Frozen glycerol stock of E. coli containing the plasmid.