HO-poly-KanMX4-HO (ATCC® 87804)

Applications: Cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations HO-poly-KanMX4-HO
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Construct size (kb): 6.06
Vector: HO-poly-KanMX4-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s): ampR
Features: promoter: HO
Cloning vector
Restriction digests of the clone give the following sizes (kb): NotI--3.0 (doublet); XbaI--6.0; PstI--5.8, 0.2.
The plasmid contains two fragments from the HO promoter inserted in pUC21-NotI polylinker along with the kanMX selectable marker. It allows for targeted integration at HO locus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

David J Stillman, personal communication

Shipped frozen
Shipping Information Frozen glycerol stock of E. coli containing the plasmid.