pUC21-NotI (ATCC® 87802)

Applications: cloning vector  /  Depositors: DJ Stillman

Designations pUC21-NotI
Permits and Restrictions

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Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 3.2500000000000000
Vector: pUC21-NotI (plasmid)
Construction: pUC21deltaBB and pFA6b
Construct size (kb): 3.25
cloning vector
Restriction digests of the clone give the following sizes (kb): PvuI--2.0, 1.2; PstI--3.25; XbaI--3.25.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)

Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257

Wach A, et al. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.. Yeast 10(13): 1793-1808, 1994. PubMed: 7747518

Shipped freeze-dried