pFSEC (ATCC® 87791)

Applications: cloning vector  /  Depositors: EW Miles

Designations pFSEC
Permits and Restrictions

View Permits

Depositors EW Miles
Biosafety Level 1
Vector Information
Size (kb): 5.6799998283386230
Vector: pFSEC (plasmid)
Promoters: Promoter Ptrc
Construction: pSTR4-2
Construct size (kb): 5.679999828338623
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
cloning vector
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3.
Overproduces yeast cystathionine beta-synthase (full length).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

Jhee KH, et al. Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein. J. Biol. Chem. 275: 11541-11544, 2000. PubMed: 10766767

Related Products
component of:ATCC 87809
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.