pFSEC (ATCC® 87791)

Applications: cloning vector  /  Depositors: EW Miles

Permits and Restrictions

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Designations pFSEC
Depositors EW Miles
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.6799998283386230
Vector: pFSEC (plasmid)
Promoters: Promoter Ptrc
Construction: pSTR4-2
Construct size (kb): 5.679999828338623
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
cloning vector
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3.
Overproduces yeast cystathionine beta-synthase (full length).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

Jhee KH, et al. Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein. J. Biol. Chem. 275: 11541-11544, 2000. PubMed: 10766767

Related Products
component of:ATCC 87809
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.