pEBA-10 (ATCC® 87788)

Applications: contains sequence  /  Depositors: EW Miles

Permits and Restrictions

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Designations pEBA-10
Depositors EW Miles
Biosafety Level 1
Vector Information
Size (kb): 6.179
Vector type: plasmid
Promoter: Ptrc
Construction: pCR2
Marker(s): TRP1, ampR
Construct size (kb): 6.179
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
Transcription terminator: rrnBT1, rrnBT2
Applications
contains sequence
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1; PstI--6.1; EcoRI/PstI--4.0, 2.1.
Overproduces the alpha2 and beta2 subunit of tryptophan synthase from Salmonella typhimurium.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843

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