pEBA-10 (ATCC® 87788)

Applications: contains sequence  /  Depositors: EW Miles

Permits and Restrictions

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Designations pEBA-10
Depositors EW Miles
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.179
Vector type: plasmid
Promoter: Ptrc
Construction: pCR2
Marker(s): TRP1, ampR
Construct size (kb): 6.179
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
Transcription terminator: rrnBT1, rrnBT2
contains sequence
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1; PstI--6.1; EcoRI/PstI--4.0, 2.1.
Overproduces the alpha2 and beta2 subunit of tryptophan synthase from Salmonella typhimurium.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843

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