pCu425CUP1 (ATCC® 87728)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

Designations pCu425CUP1
Permits and Restrictions

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Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 7.3359999656677250
Vector: pCu425CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS425, CUP1 promoter
Construct size (kb): 7.335999965667725
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
cloning vector
Restriction digests of the vector give the following sizes (kb): SacI--7.3; SacI/SpeI--7.0, 0.26.
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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