Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 6.0770001411438000
Vector: pZilch (phagemid)
Promoters: Promoter CMV
Construct size (kb): 6.0770001411438
Features: marker(s): ampR
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40 ori
coding sequence: mutant pheS gene
epitope tag: FLAG
encodes removable tag for protein isolation
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.
The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.
Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383