pZilch (ATCC® 87646)

Applications: encodes removable tag for protein isolationexpression vectorvector permitting construction of fusion proteinsvector permitting positive selection for insertsvector permitting production of single-stranded DNA  /  Depositors: C Hovens

Designations pZilch
Permits and Restrictions

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Depositors C Hovens
Biosafety Level 1
Vector Information
Size (kb): 6.0770001411438000
Vector: pZilch (phagemid)
Promoters: Promoter CMV
Construction: pCATCH
Construct size (kb): 6.0770001411438
Features: marker(s): ampR
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: BamHI...XhoI
MCS: BglII...SalI
terminator: SV40
coding sequence: mutant pheS gene
epitope tag: FLAG
encodes removable tag for protein isolation
expression vector
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.
The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383

Shipped freeze-dried