pZilch (ATCC® 87646)

Applications: encodes removable tag for protein isolationexpression vectorvector permitting construction of fusion proteinsvector permitting positive selection for insertsvector permitting production of single-stranded DNA  /  Depositors: C Hovens

Permits and Restrictions

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Designations pZilch
Depositors C Hovens
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.0770001411438000
Vector: pZilch (phagemid)
Promoters: Promoter CMV
Construction: pCATCH
Construct size (kb): 6.0770001411438
Features: marker(s): ampR
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: BamHI...XhoI
MCS: BglII...SalI
terminator: SV40
coding sequence: mutant pheS gene
epitope tag: FLAG
encodes removable tag for protein isolation
expression vector
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.
The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Schneider S, et al. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197: 337-341, 1997. PubMed: 9332383

Shipped freeze-dried