Size (kb): 8.3000001907348630
Vector: pEA500 (plasmid)
Promoters: Promoter T3
Construction: pEA2, pSP2
Construct size (kb): 8.300000190734863
Features: insert detection: lacZ'
promoter: T7, T3
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--8.3; XhoI--8.3; HindIII--5.3, 1.6, 1.4.
Plasmid pEA500 is a cloning vector that carries his7+ and ars1, yet retains the ability to use the blue/white color screen to identify recombinants.
The vector was constructed by inserting the PstI-SmaI fragment of pEA2 into plasmid pSP2 digested with NsiI and StuI (both recognize unique sites within the URA3 gene).
Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169