Size (kb): 4.2500000000000000
Vector: pPE1000 (phagemid)
Promoters: Promoter duplicated CaMV35S
Construction: pGEM-9Zf derivative pART7
Construct size (kb): 4.25
Features: marker(s): ampR
promoter: duplicated CaMV35S
replicon: pMB1, f1 ori
transcription terminator: from the nopaline synthase (NOS) gene
epitope tag: HA1 epitope (YPYDVPDYA)
encodes an epitope tag for protein isolation or monitoring
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): KpnI--2.9, 1.3; NotI--2.9, 1.3; XhoI--4.2.
Restriction sites flanking the 2X35S/EPITOPE/NOS 3' cassette allow excision with either NotI for subcloning into the binary vector pART27 or KpnI for subcloning into the binary v ector pRD400.
There are also unique SacI and XhoI sites for either directional subcloning or insertion of other plant transgenes before subcloning.
The vector contains a specialized cassette allowing the in-frame N- or C-terminal fusion of a polymerase chain reaction (PCR)-amplified mature protein coding sequence from any gene to a sequence encoding an epitope of the influenza hemagglutanin a-chain.
The vector was constructed using the pGEM-9Zf derivative pART7 from the vector PJIT166 and the 3' transcription terminator from the nopaline synthase (NOS) gene subcloned from the vector pCaGUS.
Hancock KR, et al. pPE1000: a versatile vector for the expression of epitope-tagged foreign proteins in transgenic plants. BioTechniques 22: 861-865, 1997. PubMed: 9149864