pPE1000 (ATCC® 87573)

Applications: encodes an epitope tag for protein isolation or monitoringvector useful for cloning PCR products  /  Depositors: PM Ealing

Permits and Restrictions

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Designations pPE1000
Depositors PM Ealing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.2500000000000000
Vector: pPE1000 (phagemid)
Promoters: Promoter duplicated CaMV35S
Construction: pGEM-9Zf derivative pART7
Construct size (kb): 4.25
Features: marker(s): ampR
promoter: duplicated CaMV35S
replicon: pMB1, f1 ori
MCS: NcoI...BglII
MCS: SalI...EcoRI
transcription terminator: from the nopaline synthase (NOS) gene
epitope tag: HA1 epitope (YPYDVPDYA)
encodes an epitope tag for protein isolation or monitoring
vector useful for cloning PCR products
Restriction digests of the clone give the following sizes (kb): KpnI--2.9, 1.3; NotI--2.9, 1.3; XhoI--4.2.
Restriction sites flanking the 2X35S/EPITOPE/NOS 3' cassette allow excision with either NotI for subcloning into the binary vector pART27 or KpnI for subcloning into the binary v ector pRD400.
There are also unique SacI and XhoI sites for either directional subcloning or insertion of other plant transgenes before subcloning.
The vector contains a specialized cassette allowing the in-frame N- or C-terminal fusion of a polymerase chain reaction (PCR)-amplified mature protein coding sequence from any gene to a sequence encoding an epitope of the influenza hemagglutanin a-chain.
The vector was constructed using the pGEM-9Zf derivative pART7 from the vector PJIT166 and the 3' transcription terminator from the nopaline synthase (NOS) gene subcloned from the vector pCaGUS.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Hancock KR, et al. pPE1000: a versatile vector for the expression of epitope-tagged foreign proteins in transgenic plants. BioTechniques 22: 861-865, 1997. PubMed: 9149864

Shipped freeze-dried