Size (kb): 9.4689998626708980
Vector: pSON8 (plasmid)
Promoters: Promoter ADH
Construct size (kb): 9.468999862670898
Features: marker(s): URA3
replicon: 2 micron ori
coding sequence: SOS (human Son of sevenless)
vector component of two-hybrid screening system
Restriction digests of the clone give the following sizes (kb): HindIII--6.0, 3.5; XbaI--9.5; BamHI--9.5.
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.
The vector contains the human SOS gene with a polylinker on the N-terminus to attach "bait" gene for use in SOS Recruitment System.
Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808