pSON8 (ATCC® 87564)

Applications: vector component of two-hybrid screening system  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pSON8
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.4689998626708980
Vector: pSON8 (plasmid)
Promoters: Promoter ADH
Construction: pUC
Construct size (kb): 9.468999862670898
Features: marker(s): URA3
marker(s): ampR
promoter: ADH
replicon: 2 micron ori
replicon: pMB1
MCS: NcoI...EagI
terminator: ADH
coding sequence: SOS (human Son of sevenless)
vector component of two-hybrid screening system
Restriction digests of the clone give the following sizes (kb): HindIII--6.0, 3.5; XbaI--9.5; BamHI--9.5.
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.
The vector contains the human SOS gene with a polylinker on the N-terminus to attach "bait" gene for use in SOS Recruitment System.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

Shipped freeze-dried