pRS415 (ATCC® 87520)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS415
Depositors P Hieter
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.02
Vector: pRS415 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):LEU2,ampR
Construct size (kb): 6.02
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--5.55, 0.45; BglI--4.3, 1.7; ClaI--4.45, 1.55.
A YC-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Related Products
component of:ATCC 87533
Shipping Information

 Frozen glycerol stock of phagemid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87520