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Host
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Distribution host: Escherichia coli HB101 ( ATCC 33694) |
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Vector Information
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Size (kb): 5.407 DESCRIPTION OF VECTOR:
Intact vector size: 5.407
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI
XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV ClaI SalI XhoI ApaI KpnI
Construction: pRS401, pRS84
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): MET15, ->, 421-1270
replicon: f1, <-, 1860-2316
promoter for in vitro transcription: T7, ->, 2486-2505
MCS: KpnI...SacI, ->, 2513-2615
promoter for in vitro transcription: T3, <-, 2632-2651
coding sequence: lacZ', <-, 2317-2676
promoter: lac, <-, 2721-2749
replicon: pMB1, 3075-3075
marker(s): ampR, <-, 3833-4693
centromere: CEN6, 4835-4951
replicon: ARS4, 4952-5353 Vector: pRS411 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS401, pRS84 Marker(s):ampR,MET15 Construct size (kb): 5.407 Features: marker(s): MET15
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
coding sequence: lacZ'
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Applications
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YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
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References
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Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447
Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
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Related Products
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component of: ATCC 87476
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