p34E-Tp plasmid in E. coli (ATCC® 87447)

Applications: contains easily purifiable cassette(s) for construction dihydrofolate reductase, trimethoprim resistanceproduces protein dihydrofolate reductase, trimethoprim resistance  /  Depositors: DE Woods, D DeShazer

Designations p34E-Tp plasmid in E. coli
Depositors DE Woods, D DeShazer
Biosafety Level 1
Vector Information
Vector: p34E-Tp (plasmid)
Promoters: Promoter for in vitro transcription SP6
Construction: pGEM-3, pGEM-4, dhfrIIb (tmpR)
Marker(s):ampR,tmpR
Construct size (kb): 3.527
Features: marker(s): ampR
marker(s): tmpR
promoter for expression: lac
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
MCS: EcoRI...HindIII
MCS: HindIII...EcoRI
Applications
contains easily purifiable cassette(s) for construction dihydrofolate reductase, trimethoprim resistance
produces protein dihydrofolate reductase, trimethoprim resistance
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 0.4, 0.2; BglI--3.5; EcoRI--2.9, 0.6.
Plasmid containing a trimethoprim resistance cassette useful as a selectable marker, for cassette mutagenesis of cloned genes, and for the construction of new cloning vectors.
Trimethoprim insert contains nucleotides 399-1016 of GenBank J01774 and was cloned into the EcoRI site of the pGEM-3/4 vector.
Media ATCC® Medium 2031: LB + 1.5 mg/ml trimethoprim
Growth Conditions
Temperature: 37.0°C
References

DeShazer D, Woods DE. Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. BioTechniques 20: 762-764, 1996. PubMed: 8723912

Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid