p34E-Tp plasmid in E. coli (ATCC® 87447)

Applications: contains easily purifiable cassette(s) for construction dihydrofolate reductase, trimethoprim resistanceproduces protein dihydrofolate reductase, trimethoprim resistance  /  Depositors: DE Woods, D DeShazer

Permits and Restrictions

View Permits

Designations p34E-Tp plasmid in E. coli
Depositors DE Woods, D DeShazer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Vector: p34E-Tp (plasmid)
Promoters: Promoter for in vitro transcription SP6
Construction: pGEM-3, pGEM-4, dhfrIIb (tmpR)
Marker(s):ampR,tmpR
Construct size (kb): 3.527
Features: marker(s): ampR
marker(s): tmpR
promoter for expression: lac
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
MCS: EcoRI...HindIII
MCS: HindIII...EcoRI
Applications
contains easily purifiable cassette(s) for construction dihydrofolate reductase, trimethoprim resistance
produces protein dihydrofolate reductase, trimethoprim resistance
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 0.4, 0.2; BglI--3.5; EcoRI--2.9, 0.6.
Plasmid containing a trimethoprim resistance cassette useful as a selectable marker, for cassette mutagenesis of cloned genes, and for the construction of new cloning vectors.
Trimethoprim insert contains nucleotides 399-1016 of GenBank J01774 and was cloned into the EcoRI site of the pGEM-3/4 vector.
Media ATCC® Medium 2031: LB + 1.5 mg/ml trimethoprim
Growth Conditions
Temperature: 37.0°C
References

DeShazer D, Woods DE. Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. BioTechniques 20: 762-764, 1996. PubMed: 8723912

Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid