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Host
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Distribution host: Escherichia coli HB101 ( ATCC 33694) |
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Vector Information
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Size (kb): 7.7470002174377440 DESCRIPTION OF VECTOR:
Intact vector size: 7.747
Type of vector: plasmid
Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS415
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3452
promoter for expression: ADH, <-, 3452-4952
marker(s): ampR, <-, 6199-7129
replicon: CEN6/ARSH4, ->, 7234-7747 Vector: p415 ADH (plasmid) Promoters: Promoter for expression ADH Construction: pRS415 Marker(s):LEU2,ampR Construct size (kb): 7.747000217437744 Features: marker(s): LEU2
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
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Applications
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YX-type (expression) shuttle vector vector with low copy number
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Comments
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Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.0, 1.5; EcoRI--5.4, 2.3; KpnI--5.8, 1.7. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Low copy number shuttle expression vector.
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References
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Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
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Shipping Information
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Distributed: freeze-dried
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