p416 MET25 (ATCC® 87324)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Designations p416 MET25
Permits and Restrictions

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Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 5.5050001144409180
Vector: p416 MET25 (plasmid)
Promoters: Promoter for expression MET25
Construction: pRS416, MET25 promoter
Construct size (kb): 5.505000114440918
Features: marker(s): URA3
marker(s): ampR
promoter for expression: MET25
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.4; EcoRV--3.8, 1.7; PstI--3.6, 1.9.
Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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