p416 MET25 (ATCC® 87324)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p416 MET25
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5050001144409180
Vector: p416 MET25 (plasmid)
Promoters: Promoter for expression MET25
Construction: pRS416, MET25 promoter
Construct size (kb): 5.505000114440918
Features: marker(s): URA3
marker(s): ampR
promoter for expression: MET25
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.4; EcoRV--3.8, 1.7; PstI--3.6, 1.9.
Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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