Size (kb): 3.0000000000000000
Vector: pUMA33 (plasmid)
Promoters: Promoter for in vitro transcription SP6
Construct size (kb): 3.0
Features: initiation codon: ATG
promoter for in vitro transcription: SP6
restriction site: NcoI
signal peptide coding region: NCAM
stop codons: 3
construct to generate mRNA that can be expressed in Xenopus oocytes
vector for constructing cDNA libraries
vector permitting RNA synthesis in vitro
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI--3.0; EcoRI--3.0; XbaI--3.0.
Vector designed for in vitro transcription of cloned inserts encoding open reading frames. Transcribed RNA encodes the murine NCAM signal sequence for protein export and will contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.
Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614