pUMA33 (ATCC® 87304)

Applications: construct to generate mRNA that can be expressed in Xenopus oocytesvector for constructing cDNA librariesvector permitting RNA synthesis in vitrovector with sequences for protein export  /  Depositors: UA Heinlein

Permits and Restrictions

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Designations pUMA33
Depositors UA Heinlein
Biosafety Level 1
Vector Information
Size (kb): 3.0000000000000000
Vector: pUMA33 (plasmid)
Promoters: Promoter for in vitro transcription SP6
Construction: pSP64
Marker(s):ampR
Construct size (kb): 3.0
Features: initiation codon: ATG
marker(s): ampR
promoter for in vitro transcription: SP6
replicon: pMB1
MCS: EcoRI...NotI
restriction site: NcoI
signal peptide coding region: NCAM
stop codons: 3
Applications
construct to generate mRNA that can be expressed in Xenopus oocytes
vector for constructing cDNA libraries
vector permitting RNA synthesis in vitro
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.0; EcoRI--3.0; XbaI--3.0.
Vector designed for in vitro transcription of cloned inserts encoding open reading frames. Transcribed RNA encodes the murine NCAM signal sequence for protein export and will contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614

Shipped freeze-dried
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