pTM-N (ATCC® 87197)

Applications: expression vectorvector with sequences for protein export  /  Depositors: X Liu

Designations pTM-N
Permits and Restrictions

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Depositors X Liu
Biosafety Level 1
Vector Information
Size (kb): 2.8499999046325680
Vector: pTM-N (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pET-3a,pIN-III-ompA3
Construct size (kb): 2.849999904632568
Features: marker(s): ampR
other: sequence for 3' mRNA secondary structure
other: sequence for 5' mRNA secondary structure
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NcoI...BamHI
ribosome-binding site: ompA Shine-Dalgarno
signal peptide coding region: ompA
expression vector
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI--2.9; EcoRI--2.9; NcoI--2.9.
Expression vector permitting overproduction and secretion of foreign proteins in an unfused state. The ompA signal sequence directs proteins to the periplasmic space.
pTM-N differs from pTO-N (GenBank accession M34008) only in the bla (ampR) gene. The PstI site in M34008 has been removed in pTM-N.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Deng TL, et al. A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli: overproduction of bovine pancreatic phospholipase A2. Gene 93: 229-234, 1990. PubMed: 2227436

Shipped freeze-dried