Size (kb): 7.6999998092651370
Vector: pIIIEx423 RPR (plasmid)
Promoters: Promoter for in vitro transcription T7
Construct size (kb): 7.699999809265137
Features: insert detection: lacZ'
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
YE-type (episomal) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): HindIII--2.2, 5.6; BamHI--7.7; EcoRI--7.7.
Cloned inserts can be transcribed from the S. cerevisiae RNase P RNA gene (RPR1) intragenic promoter to produce RPR1 leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167
- 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS423.
Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876