pIIIEx423 RPR (ATCC® 87172)

Applications: YE-type (episomal) shuttle vectorvector permitting RNA synthesis in vitrovector permitting expression of small structured RNAsvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: DR Engelke

Permits and Restrictions

View Permits

Designations pIIIEx423 RPR
Depositors DR Engelke
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.6999998092651370
Vector: pIIIEx423 RPR (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS423
Marker(s):ampR,HIS3
Construct size (kb): 7.699999809265137
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: RPR1
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
terminator: RPR1
Applications
YE-type (episomal) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--2.2, 5.6; BamHI--7.7; EcoRI--7.7.
Cloned inserts can be transcribed from the S. cerevisiae RNase P RNA gene (RPR1) intragenic promoter to produce RPR1 leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS423.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet