Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 7.6999998092651370
Vector: pIIIEx423 RPR (plasmid)
Promoters: Promoter for in vitro transcription T7
Construct size (kb): 7.699999809265137
Features: insert detection: lacZ'
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
YE-type (episomal) shuttle vector
vector permitting RNA synthesis in vitro
vector permitting expression of small structured RNAs
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): HindIII--2.2, 5.6; BamHI--7.7; EcoRI--7.7.
Cloned inserts can be transcribed from the S. cerevisiae RNase P RNA gene (RPR1) intragenic promoter to produce RPR1 leaders fused to RNA corresponding to inserted sequences.
One of a series of shuttle vectors (ATCC 87167
- 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.
Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS423.
Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876