pKSM717 (ATCC® 87162)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting construction of fusion proteinsvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

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Designations pKSM717
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5840001106262210
Vector: pKSM717 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC18
Construct size (kb): 3.584000110626221
Features: insert detection: lacZalpha
marker(s): ampR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: DsaI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
expression vector
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--2.86; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.11; PvuII--0.48.
Differs from pKSM718 (ATCC 87163) only in the orientation of the ampicillin resistance gene.
Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried